文章标题：Effect of α-momordicine on proliferation ａnd apoptosis of liver cancer, ａnd its associated mechanisms of action
作者列表：Mei Yang, Wen Jin, Wei Shi, Bo Wang, Qing Li, Chunfeng Guan
期刊：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
发表时间：2021-6-29
影响因子：0.533
DOI:10.4314/tjpr.v18i9.22
主要研究成果：Abstract
Purpose: To investigate the effect of α-momordicine （α-MMC） on liver cancer cell proliferation ａnd apoptosis, ａnd to elucidate the mechanisms of action involved.
Methods: In in vitro experiments, hepatoma cell lines were used, while nude mice with hepatocellular carcinoma were used for in vivo studies. Cancer cell proliferation was determined using MTT assay while apoptosis was assayed by flow cytometry ａnd TUNNEL staining. Gene expression was determined with real-time polymerase chain reaction （RT-PCR）, while protein expression levels were assayed by Western blot, immunohistochemistry ａnd immunofluorescence.
Results: Alpha-MMC decreased HCC cell viability dose-dependently （p < 0.05）. In HepG2 cells, G2/M cell cycle was halted after 48 h intervention with 1.24 mg/mL α-MMC. However, at G0/G1 phase, αMMC at doses of 1.06 ａnd 0.92 mg/mL caused cell cycle arrest of HCC-LM3 ａnd SMMC-7721 cells. In vivo studies showed that after establishment of the nude mice liver cancer model, exposure to α-MMC at a dose of 0.70 mg/kg or 2.08mg/kg for 4 weeks reduced the size of liver cancer in the treatment group, relative to control group; mean diameter of liver cancer decreased from 2.16 to 0.51 cm, while mean volume decreased from 1.185 to 0.085 cm3 . Moreover, α-MMC increased apoptosis level in liver cancer tissues in nude mice, ａnd down-regulated the expressions of P-AKT, RAGE, MMP-9 ａnd HMGB1, but upregulated Bax/Bcl2 ratio （p < 0.05）.
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